AnemoClear Project
AnemoClear: An optimized tissue-clearing protocol to explore Exaiptasia pallida internal structures.
Exploration of the symbiotic relationships between different marine organisms and their resident algae arises as a pivotal field in the study of complex marine beings, including corals and sponges. In this respect, Exaiptasia pallida (Ep) strain - CC7 - is the model of choice due to its symbiotic behavior, its accessibility, and the possibility of cloning. Our AnemoClear procedure allows for the first time to visualize in the whole Ep the internal tissues, including stinging filaments, siphonoglyph, and mesenteries with their fixation point. This protocol has the potential not only to explore the structure of Ep tissues under physiological conditions but also the interaction between Exaiptasia pallida and its symbiotic algae.
This project is a collaboration between the C3M and the Scientific Center of Monaco (CSM).
The teams involved in the "AnemoClear" project:
C3M: Jérôme Gilleron.
CSM: Dorota Czerucka, Mélanie Billaud (PhD student).
Exploration of the symbiotic relationships between different marine organisms and their resident algae arises as a pivotal field in the study of complex marine beings, including corals and sponges. In this respect, Exaiptasia pallida (Ep) strain - CC7 - is the model of choice due to its symbiotic behavior, its accessibility, and the possibility of cloning. Our AnemoClear procedure allows for the first time to visualize in the whole Ep the internal tissues, including stinging filaments, siphonoglyph, and mesenteries with their fixation point. This protocol has the potential not only to explore the structure of Ep tissues under physiological conditions but also the interaction between Exaiptasia pallida and its symbiotic algae.
This project is a collaboration between the C3M and the Scientific Center of Monaco (CSM).
The teams involved in the "AnemoClear" project:
C3M: Jérôme Gilleron.
CSM: Dorota Czerucka, Mélanie Billaud (PhD student).